Applejack speaks with a Southern American accent, a thick southern drawl, similar to the accent of the southwestern Missouri and Oklahoma Ozarks, where there is a great number of apple orchards. In numerous episodes, she refers to members of her friends and family as "sugarcube" as a sign of affection.
Applejack dislikes things that are "girly", like the froufrou dresses in Suited for Success. Applejack describes in a flashback in The Cutie Mark Chronicles how she tried being a pony of elegance for a short time as a young filly by moving to Manehattan to live with her Aunt and Uncle Orange, but ultimately decided to return to the farm after she realized that she was homesick. When Apple Bloom starts speaking French in The Cutie Pox, Applejack says her sister is "speakin' in fancy".
Applejack possesses incredible physical strength due to, as she explains in Fall Weather Friends, years of applebucking, the practice of harvesting apple trees by kicking them to knock the apples down, which is first named in Applebuck Season.
Not much is known about Applejack's parents, but she lives with her grandmother, Granny Smith. Her brother is called Big McIntosh and her little sister is Apple Bloom. She also is related to her aunt and uncle, The Oranges, who live in Manehattan. In the episode Pinkie Apple Pie it is alluded that Pinkie Pie is one of her distant relatives. She has a pet dog named Winona and in the episode Apple Family Reunion, she shown to have relatives from all over Equestria.
Applejack gained her cutie mark in the episode called, Call of the Cutie. Applejack had left her farm to go live with her aunt and uncle in Manehattan. There Applejack becomes very homesick, and while looking out her window she sees a sonic rainboom. The rainboom convinces Applejack to head home to her family. Back at the farm she realizes it is where she truly belongs and receives her cutie mark of three apples.